Journal: bioRxiv

Article Title: Matrix mechanics governs mechano-metabolic adaptation across cancer grades in bladder spheroids

doi: 10.64898/2025.12.03.692076

Figure Lengend Snippet: Spheroids representing three bladder cancer cell lines (HCV29, T24, HT1376) were embedded in collagen–hyaluronan (Col–HA) hydrogels. Relative expressions of E-cadherin, N-cadherin, hexokinase 2 (HK2), and syndecan-4 (SDC4) were normalized to control (i.e., expressions in spheroids grown only in culture medium, n = 3) and converted to fold change. Statistical significance was obtained by unpaired Student’s t-test at the level of 0.05 (* p < 0.01, **p <0.001, ***p <0.0001).

Article Snippet: Roswell Park Memorial Institute 1640 culture medium (RPMI, Merck, Poland) supplemented with 10% Fetal Bovine Serum (FBS, ATCC, USA) was used for HCV29 and T24 cells, and Eagle’s Minimum Essential Medium (EMEM, ATCC, USA) with 10% FBS for HT1376 cells.

Techniques: Control

Journal: Current issues in molecular biology

Article Title: Acidic Microenvironment Enhances Cisplatin Resistance in Bladder Cancer via Bcl-2 and XIAP.

doi: 10.3390/cimb47010043

Figure Lengend Snippet: Figure 1. HEK293, T24, and HT1376 cell lines were subjected to survival assays to assess their responses to varying extracellular pH levels. Cells were plated in 96-well plates at a density of 2 × 104 cells/mL and incubated for 72 h under different pH conditions. Low pH significantly inhibited the growth of normal HEK293 cells, while bladder cancer (BC) cells exhibited resistance to low pH. Notably, HT1376 cells were less sensitive to pH changes compared to T24 cells. Data are presented as mean ± SD from four independent experiments.

Article Snippet: We used the BC cell lines HT1376 and T24, as well as human embryonic kidney 293 (HEK293) cells, purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Incubation

Journal: Current issues in molecular biology

Article Title: Acidic Microenvironment Enhances Cisplatin Resistance in Bladder Cancer via Bcl-2 and XIAP.

doi: 10.3390/cimb47010043

Figure Lengend Snippet: Figure 3. Effects of combinatory treatment with cisplatin (CDDP) and autophagy inhibitor chloro- quine, or BCL-2 inhibitor navitoclax, at varying pH levels on cell viability. The time-dependent changes at 0 h, 24 h, 48 h, and 72 h of culturing are shown in each graph. CDDP was used at a concen- tration of 2 µM. Relative cell viability was assessed using the MTS assay after 72 h. Two asterisks (**) indicate that the p-value is less than 0.01, ns indicates no significant difference. (a) Chloroquine was administered at concentrations of 15 µM and 30 µM. Under neutral pH conditions, chloroquine inhib- ited HT1376 growth in a concentration-dependent manner, both with and without CDDP; this effect was not observed in an acidic environment. (b) Navitoclax was administered at concentrations of 2 µM and 4 µM. Bladder cancer (BC) cells cultured under acidic conditions showed reduced survival with navitoclax treatment, while no significant effect was noted under neutral pH conditions.

Article Snippet: We used the BC cell lines HT1376 and T24, as well as human embryonic kidney 293 (HEK293) cells, purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: MTS Assay, Inhibition, Concentration Assay, Cell Culture

Journal: mAbs

Article Title: Discovery of a novel highly specific, fully human PSCA antibody and its application as an antibody-drug conjugate in prostate cancer.

doi: 10.1080/19420862.2024.2387240

Figure Lengend Snippet: Figure 1. Discovery and characterization of the PSCA antibody fab G7.(a) fab G7 binding to recombinant PSCA-Fc protein measured by ELISA. Bovine serum albumin (BSA) was used as a negative control. Experiments were performed in duplicate and the error bars denote ± SD, n = 2. (b) Kinetics of fab G7 binding to PSCA-Fc, as measured by Blitz. (c) Fab G7 binding to PSCA positive (PC-3-PSCA, Du-145-PSCA and HT1376 cells) and PSCA negative cells (PC-3, Du-145 and CHO-K1 cells) as tested by flow cytometry. An irrelevant fab (anti-SARS-CoV-2 fab ab1) was used as the isotype control. Fab G7 at the concentration of 500 nM was incubated with cells. (d-g) competition of fab G7 and fab F12 binding to HT1376 cell surface-associated PSCA by the recombinant PSCA-Fc protein (d and f) and by the murine PSCA antibody 7F5 (e and g). 500 nM of fab G7 or 200 nM of F12 was incubated with cells in the presence of gradient concentration of competitors. The bound fab G7 or F12 was detected by the pe-conjugated anti-flag tag antibody.

Article Snippet: The prostate cancer cell lines PC-3 and Du-145 and the bladder cancer cell-line HT1376 were purchased from the American Type Culture Collection (ATCC).

Techniques: Binding Assay, Recombinant, Enzyme-linked Immunosorbent Assay, Negative Control, Flow Cytometry, Control, Concentration Assay, Incubation, FLAG-tag